Evaluation of Enrichment Techniques for Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
Immunoaffinity Enrichment and Mass Spectrometry Analysis of Protein Methylation
Protein methylation is a common posttranslational modification that mostly occurs on arginine and lysine residues. Arginine methylation has been reported to regulate RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Lysine methylation is best known to regulate histone function and is involved in epigenetic regulation of gene transcription. To...
متن کاملGas Chromatography Mass Spectrometry Coupling Techniques
Relative to other metabolomics analysis techniques, gas chromatography mass spectrometry (GC/MS) is one of the earliest applied analysis techniques in metabolomics. The first paper on metabolomics (metabolic profiling) is derived from the application of GC/MS analysis in urine and tissue extracts (Dalgliesh et al. 1966). With the arrival of omics era and the proposing of metabolomics concept, p...
متن کاملEvaluation of peak-picking algorithms for protein mass spectrometry.
Peak picking is an early key step in MS data analysis. We compare three commonly used approaches to peak picking and discuss their merits by means of statistical analysis. Methods investigated encompass signal-to-noise ratio, continuous wavelet transform, and a correlation-based approach using a Gaussian template. Functionality of the three methods is illustrated and discussed in a practical co...
متن کاملAntibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers.
A major bottleneck for validation of new clinical diagnostics is the development of highly sensitive and specific assays for quantifying proteins. We previously described a method, stable isotope standards with capture by antipeptide antibodies, wherein a specific tryptic peptide is selected as a stoichiometric representative of the protein from which it is cleaved, is enriched from biological ...
متن کاملAccurate Protein Complex Retrieval by Affinity Enrichment Mass Spectrometry (AE-MS) Rather than Affinity Purification Mass Spectrometry (AP-MS)*
Protein-protein interactions are fundamental to the understanding of biological processes. Affinity purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation. Previously, complexes were purified as much as possible, frequently followed by identification of individual gel bands. However, todays mass spectrometers are highly sensitive, and powe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2007
ISSN: 1525-1578
DOI: 10.2353/jmoldx.2007.060031